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fbs cell culture media  (Biowest SAS)

 
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    Biowest SAS fbs cell culture media
    Fbs Cell Culture Media, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fbs+cell+culture+media/fbs+cell+culture+media/pm40509555-62-7-8
    Average 90 stars, based on 1 article reviews
    fbs cell culture media - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.

    Virus:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.

    Recombinant:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.

    Multiplex sample analysis:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.

    Magnetic Beads:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.

    Membrane:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.

    Concentration Assay:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.

    Incubation:

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).


    Article Title: ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-PPP6R2 Santa Cruz Biotechnology Cat#sc-376238; RRID:AB_10986121 Mouse monoclonalanti-STRN Santa Cruz Biotechnology Cat#sc-136084; RRID:AB_2271282 Rabbit polyclonal anti-STRN4 MERCK Cat#SAB1302464 Rabbit polyclonal anti-LC3A/B Cell Signaling Technology Cat#4108S; RRID:AB_2137703 Rabbit monoclonal anti-ATG14-pS29 Cell Signaling Technology Cat#92340S; RRID:AB_2800182 Rabbit monoclonal anti-ATG14 Cell Signaling Technology Cat#96752; RRID:AB_2737056 Rabbit polyclonal anti-RPS6KB1-pT389 Cell Signaling Technology Cat#9205; RRID:AB_330944 Rabbit polyclonal anti-ERK1/2-pT201/Y204 Cell Signaling Technology Cat#9101S; RRID:AB_331646 Mouse monoclonal anti-b-Actin Santa Cruz Biotechnology Cat#sc-47778; RRID:AB_2714189 Peroxidase-conjugated goat anti-rabbit IgG Jackson Immuno Research Cat#111-035-045; RRID:AB_2337938 Peroxidase-conjugated goat anti-mouse IgG Jackson Immuno Research Cat#115-035-062; RRID:AB_2338504 guinea pig anti-SQSTM1 Progen Cat#GP62-C; RRID:AB_2687531 anti-guinea pig Invitrogen Cat#A11073; RRID:AB_2534117 Bacterial and virus strains E. coli DH5a CGSC Cat#12384 Chemicals, peptides, and recombinant proteins DMEM PAN Biotech Cat#P04-04510 SILAC-DMEM PAN Biotech Cat#P04-02505 DPBS PAN Biotech Cat#P04-36500 HBSS GIBCO Cat#14025100 FBS Biowest Cat#S181B-500 Dialyzed FBS Biowest Cat#S181D-500 Penicillin/streptomycin PAN Biotech Cat#P06-07100 GlutaMAX GIBCO Cat#35050038 Trypsin for cell culture PAN Biotech Cat#P10-023100 Pierce Anti-HA Magnetic Beads Thermo Scientific Cat#88837 Strep-Tactin XT 4Flow IBA Cat#2-5030-010 Nitrocellulose membrane 0.45 mm Amersham Cat#GE10600000 PVDF membrane 0.2 mm Amersham Cat#GE10600021 TransBlot Turbo RTA Midi NC BIO-RAD Cat#1704271 TransBlot Turbo RTA Midi PVDF BIO-RAD Cat#1704273 WesternBright ECL Advansta Cat#K12049-D50 Benzonase Sigma-Aldrich Cat#1.01697.0001 Arg10 Sigma-Aldrich Cat#608033 Arg6 Sigma-Aldrich Cat#643440 Lys4 Sigma-Aldrich Cat#616192 Lys8 Sigma-Aldrich Cat#608041 Arg0 Sigma-Aldrich Cat#11039 Lys0 Sigma-Aldrich Cat#L8662 L-Proline Fluka Cat#81710 PhosSTOP Roche Cat#04-906-837-001 Proteases Inhibitor Cocktail Roch Cat#11-697-498-001 Rapamycin LC Laboratories Cat#R-5000 (Continued on next page) Cell Reports 36, 109762, September 28, 2021 e1

    Article Title: 2-Hydroxypropyl-β-cyclodextrin (HPβCD) as a Potential Therapeutic Agent for Breast Cancer.
    Article Snippet: All media was supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin- streptomycin (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Cancer cell invasion alters the protein profile of extracellular vesicles
    Article Snippet: PC3 cells (p9‐p16) were plated onto two 15 cm plates in RPMI media (10% FBS [S181B, Biowest], 100 U/μg/mL P/S and 2 mM L‐glut) and were grown in 37°C, 5% CO 2 until they reached 60% confluency.



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